H2N2V1, Main, Exploration, bibRecord, 002873

The localization of influenza virus in the respiratory tract of ferrets: susceptible nasal mucosa cells produce and release more virus than susceptible lung cells.

Identifieur interne : 002873 ( Main/Exploration ); précédent : 002872; suivant : 002874

The localization of influenza virus in the respiratory tract of ferrets: susceptible nasal mucosa cells produce and release more virus than susceptible lung cells.

Auteurs : D. Cavanagh ; F. Mitkis ; C. Sweet ; M H Collie ; H. Smith

Source :

The Journal of general virology [ 0022-1317 ] ; 1979.

RBID : pubmed:521800

Descripteurs français

KwdFr :
- Animaux, Carnivora (microbiologie), Furets (microbiologie), Hémagglutinines virales, Muqueuse nasale (microbiologie), Poumon (microbiologie), Techniques de culture d'organes, Trypsine (pharmacologie), Virus de la grippe A (croissance et développement), Virus de la grippe A (immunologie).
MESH :
- croissance et développement : Virus de la grippe A.
- immunologie : Virus de la grippe A.
- microbiologie : Carnivora, Furets, Muqueuse nasale, Poumon.
- pharmacologie : Trypsine.
- Animaux, Hémagglutinines virales, Techniques de culture d'organes.

English descriptors

KwdEn :
- Animals, Carnivora (microbiology), Ferrets (microbiology), Hemagglutinins, Viral, Influenza A virus (growth & development), Influenza A virus (immunology), Lung (microbiology), Nasal Mucosa (microbiology), Organ Culture Techniques, Trypsin (pharmacology).
MESH :
- chemical , pharmacology : Trypsin.
- chemical : Hemagglutinins, Viral.
- growth & development : Influenza A virus.
- immunology : Influenza A virus.
- microbiology : Carnivora, Ferrets, Lung, Nasal Mucosa.
- Animals, Organ Culture Techniques.

Abstract

Infectious virus production by ferret nasal mucosa and lung organ cultures has been monitored in both tissue pieces and medium over 24 h following inoculation with an Asian (H2N2) strain of influenza virus. Freshly prepared cultures of nasal mucosa produced approx. 10-fold more virus per cell than fresh lung cultures. Also the nasal mucosa cells liberated into the medium a greater proportion (mean 31%) of the total virus produced than did fresh lung (mean 6%). Maintenance of lung explants for 24 h prior to inoculation resulted in a 20- to 100-fold increase in the amount of virus released. However, total virus production by fresh and maintained lung was similar. Trypsin did not increase the infectivity of virus released from any of the cultures, indicating that the haemagglutinin in the virus particles was cleaved. Similar results were obtained with a Hong Kong (H3N2) virus strain. Hence, one factor operating in the lower susceptibility of the lung compared with the nasal mucosa in vivo may be a lower capacity of lung cells both to produce and release influenza virus.

DOI: 10.1099/0022-1317-44-2-505
PubMed: 521800

Affiliations:

Le document en format XML

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<term>Influenza A virus (growth & development)</term>
<term>Influenza A virus (immunology)</term>
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<term>Muqueuse nasale (microbiologie)</term>
<term>Poumon (microbiologie)</term>
<term>Techniques de culture d'organes</term>
<term>Trypsine (pharmacologie)</term>
<term>Virus de la grippe A (croissance et développement)</term>
<term>Virus de la grippe A (immunologie)</term>
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<front><div type="abstract" xml:lang="en">Infectious virus production by ferret nasal mucosa and lung organ cultures has been monitored in both tissue pieces and medium over 24 h following inoculation with an Asian (H2N2) strain of influenza virus. Freshly prepared cultures of nasal mucosa produced approx. 10-fold more virus per cell than fresh lung cultures. Also the nasal mucosa cells liberated into the medium a greater proportion (mean 31%) of the total virus produced than did fresh lung (mean 6%). Maintenance of lung explants for 24 h prior to inoculation resulted in a 20- to 100-fold increase in the amount of virus released. However, total virus production by fresh and maintained lung was similar. Trypsin did not increase the infectivity of virus released from any of the cultures, indicating that the haemagglutinin in the virus particles was cleaved. Similar results were obtained with a Hong Kong (H3N2) virus strain. Hence, one factor operating in the lower susceptibility of the lung compared with the nasal mucosa in vivo may be a lower capacity of lung cells both to produce and release influenza virus.</div>
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<name sortKey="Mitkis, F" sort="Mitkis, F" uniqKey="Mitkis F" first="F" last="Mitkis">F. Mitkis</name>
<name sortKey="Smith, H" sort="Smith, H" uniqKey="Smith H" first="H" last="Smith">H. Smith</name>
<name sortKey="Sweet, C" sort="Sweet, C" uniqKey="Sweet C" first="C" last="Sweet">C. Sweet</name>
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The localization of influenza virus in the respiratory tract of ferrets: susceptible nasal mucosa cells produce and release more virus than susceptible lung cells.

The localization of influenza virus in the respiratory tract of ferrets: susceptible nasal mucosa cells produce and release more virus than susceptible lung cells.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration H2N2
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